Northern Blots for Small RNAs and MicroRNAs
نویسندگان
چکیده
منابع مشابه
Northern blots for small RNAs and microRNAs.
This protocol describes the detection of small RNAs (~10-200 nucleotides) by blot hybridization. The RNA samples, denatured in formamide, are separated by denaturing polyacrylamide gel electrophoresis. Because high-percentage polyacrylamide gels are required to separate RNAs in this size range, it is necessary to perform electrophoretic transfer to positively charged nylon membranes. After tran...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2014
ISSN: 1559-6095
DOI: 10.1101/pdb.prot080838